Single Step Purification of Ovine Luteinizing Hormone by Affinity Chromatography
نویسندگان
چکیده
منابع مشابه
Single step purification of ovine luteinizing hormone by affinity chromatography.
Affinity chromatography has been applied to the problem of specifically removing luteinizing hormone from sera used to supplement tissue culture media. Horse or fetal calf sera, passed through a column of an anti-luteinizing hormone immunoglobulin fraction linked covalently to agarose, were specifically depleted in their content of luteinizing hormone as seen by radioimmunoassay and by their ef...
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متن کاملSingle Step Purification of Novel Thermostable and Chelator Resistant Amylase from Bacillus Licheniformis RM44 by Affinity Chromatography
Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...
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The preparation of a number of agarose and polyacrylamide bead derivatives useful in the purification of proteins by afhnity chromatography is described. These techniques permit (a) the attachment of ligands to the gel through extended hydrocarbon chains which place the ligand at varying distances from the gel matrix backbone; (b) the covalent attachment of ligands to agarose or polyacrylamide ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44719-4